Immunocytochemichal analysis of 2-hydroxyisobutyryl-Histone H2B (Lys12) in Hela cells using a Polyclonal antibody (Product # PA5-112486) at a dilution of 1:15. Hela cells (treated with 30mM sodium butyrate for 4 hour) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.